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. 2015 Jul 17;10(4):2135–2141. doi: 10.3892/ol.2015.3503

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Copyright: © Zhang et al. This is an open access article distributed under the terms of a Creative Commons Attribution License.

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Figure 2. — miR-33a-5p expression is decreased in OS cells and tissues. (A) Relative expression of miR-33a-5p was evaluated by TaqMan (RT-qPCR). Human normal muscle RNAs were used as controls. miR-33a-5p expression was downregulated in OS tissues. (B) Confirmation of overexpression of miR-33a-5p in transfected cell lines by real-time PCR. OS cell lines U-2OS and MG-63 were transfected with miR-33a-5p precursor and miR-Scramble control, and the relative expression of miR-33a-5p was assessed by RT-qPCR. Values are presented as the mean ± standard deviation. miR, microRNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; OS, osteosarcoma.