Table I.

Primers for methylation-specific polymerase chain reaction.

Gene	M/U	Primer sequence (5′-3′)	Annealing temperature	Product length
GSTP1	M	(F)-TTCGGGGTGTAGCGGTCGTC	61	91
		(R)-GCCCCAATACTAAATCACGACG
	U	(F)-GATGTTTGGGGTGTAGTGGTTGTT	55	91
		(R)-CCACCCCAATACTAAATCACAACA
P16	M	(F)-TTATTAGAGGGTGGGGCGGATCGC	60	150
		(R)-CAACCCCAAACCACAACCATAA
	U	(F)-TTATTAGAGGGTGGGGTGGATTGT	55	151
		(R)-CAACCCCAAACCACAACCATAA
RIZ1	M	(F)-GGATTCGCGGTGATTTAC	69	161
		(R)-AACTCCAATCGAAAAAAACG
	U	(F)-ATGGGATTTGTGGTGATTTAT	58	161
		(R)-CTTAACTCCAATCAAAAAAAACA
RASSF1A	M	(F)-GGGTTTTGCGAGAGCGC	52	169
		(R)-CGCTAACAAACGCGAACCG
	U	(F)-GGTTTTGTGAGAGTGTGTTTAG	52	169
		(R)-CACTAACAAACACAAACCAAAC

M, methylated, U, unmethlyated.