Figure 3.
Knockdown of ING4 expression increases the production of IL6 that is induced by treatment with IL1B in MG-63 cells. A total of 2.0×105 cells were plated and transfected with small interfering (si)RNA for ING4 (20 nM). After 48 h, the cells were harvested. (A and C) mRNA expression and (B) protein expression were analyzed with quantitative polymerase chain reaction (qPCR) and ELISA, respectively. Significant differences (*P<0.05) compared with the control were calculated with an analysis of variance. ING4, inhibitor of growth family member 4; IL6, interleukin 6.