Figure 4.

Bovine myeloid antimicrobial peptide 28 (BMAP-28) prevents the transcription and translation of matrix metalloproteinase (MMP)3 and MMP9. (A-D) BMAP-28 suppressed the protein expression of (A and B) MMP3 and (C and D) MMP9 in the TT cells. The TT cells were treated with BMAP-28 (ranging from 1 to 4 µM) for 48 h, then the cells were collected and analyzed by flow cytometry. (E and F) Downregulation of MMP3 and MMP9 transcription in BMAP-28-treated TT cells. RNA extracted from TT cells treated with or without BMAP-28 (1, 2 and 4 µM) for 48 h were analyzed by quantitative polymerase chain reaction, as described in the Materials and methods section. Values are presented as the mean ± standard deviation from three separate experiments. *P<0.05 and **P<0.01 vs. control group.