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. 2015 Feb 3;3(4):e1009276. doi: 10.1080/21623996.2015.1009276

Figure 2.

Figure 2.

Knockdown of WAVE3 expression sensitizes TNBC cells to chemotherapy-driven apoptosis and cell death (immunofluorescence and immunoblotting assays). (A & C) Representative confocal images of Ctrl-sh and sh-W3 MDA-MB-231 cells stained Annexin V (A) and cleaved caspase3 (C) before and after treatment with doxorubicin (Dox). (B & D) Quantification of Annexin V staining levels (B) and Caspase 3 staining levels (D). Data are representative of 3 independent experiments (*, P <0.05; Student's t-test). (E) Western blot analysis with the indicated antibodies of cell lysates form the control shRNA (ctrl-sh)- or sh-WAVE3-expressing (W3-sh) MDA-MB-231 (A) of BT549 (B) cells after doxorubicin (Dox) or docetaxel (Doc) treatment. β-actin served as a loading control. The numbers below the cleaved Caspase 3 panel indicate the fold change of cleaved Caspase 3 levels, as compared to the untreated Ctrl-sh cells.