Figure 4.

Loss of WAVE3 expression inhibits endothelial angiogenesis that is induced by Chemotherapy. (A) Bright field microscopy of tube formation structures of HUVECs treated with the serum-free media (SFM) supplemented with VEGF-A (VEGF), SFM supplemented with VEGF-A and sFLT, a soluble VEGFR inhibitor (VEGF + sFLT), or conditioned media from MDA-MB-231 cells with the indicated treatments. (B) Quantification of tube formation under the indicated conditions. The number of closed tubes was counted in 12 different fields and plotted as the average of number of closed tubes per field ±SE . Each assay was repeated at least 3 times. (* and **, P <0.05; Student's t-test).