Figure 3.

Primary cultures of early human fetal cortical cells, prepared according to Ip et al. (2010) after 3 days in vitro are a mixture of cells expressing a variety of markers including TBR1; post-mitotic glutamatergic cortical neurons (A) PAX6; mitotic radial glia-like cells (B) DLX2 and GAD2; markers for intermediate progenitors and neurons committed to the GABAergic lineage (C,D). Protein expression was revealed by immunoperoxidase cytochemistry. Micrographs were kindly provided by Dr Nahidh Al-Jaberi.