FIG 4.

Localization of SIVmac239- and ΔGY-infected cells at necropsy in pig-tailed macaques with disease progression. SIV RNA in situ hybridization was performed at necropsy on jejunum (A and D), mesenteric lymph nodes (B and E), and brain tissue (C and F) from pig-tailed macaques IK37 infected with SIVmac239 with AIDS (top row) and GR26 infected with ΔGY (bottom row) with disease progression. Representative ISH images from necropsy samples collected from the four animals infected with SIVmac239 (IK37, IP82, GR06, and GR08) and the two animals that progressed to disease with ΔGY (GR26 and HA94) are shown. In SIVmac239 infection, numerous infected cells are diffusely scattered throughout the jejunum, lymph node, and brain (week 25). In the ΔGY-progressing animals, infected cells were not detectable in jejunum or brain and were only rarely present in mesenteric lymph nodes (arrows; week 74).