FIG 4.

E1B-55K and E4-ORF3 induce SUMO2/3-conjugated proteins in Ad5-infected cells. (A) U2OS cells were infected as indicated, and then protein lysates were collected at 36 h p.i. and dot blotting for total SUMO2/3 was performed. β-Actin is a loading control. Quantification was performed using LI-COR Odyssey software with SUMO2/3 normalized to β-actin. (B) U2OS cells were infected as indicated, and then protein lysates were collected at 36 h p.i. and immunoblotted for SUMO2/3. β-Actin is a loading control.