TABLE 3.
Structural parameters derived from the SAXS data
| Protein | Predicted mass (kDa) | SAXS mass (kDa)a | aRg (Å)b | bRg (Å)c | Dmax (Å) |
|---|---|---|---|---|---|
| GAG | 60.8 | 57.4 | 54.1 ± 1.2 | 56.1 ± 0.6 | 192 ± 3 |
| ΔMBD | 50.2 | 52.2 | 46.9 ± 1.2 | 48.8 ± 0.4 | 164 ± 3 |
| ΔFlexd | 49.1 | 51.0 | 49.6 ± 1.6 | 49.5 ± 0.9 | 172 ± 4 |
| MA | 16.4 | 19.9 | 29.6 ± 0.4 | 31.9 ± 0.3 | 116 ± 3 |
| MBD | 10.6 | 9.4 | 16.5 ± 0.6 | 17.1 ± 0.5 | 60 ± 2 |
a
Calculated using the web portal SAXS MoW (www.if.sc.usp.br/∼SAXS).
b
TheaRg value was derived from Guinier fitting, using Igor Pro (Wavemetrics, Lake Oswego, OR, USA).
c
The bRg value was derived from GNOM fitting.
d
Deletion of Gag amino acids 104 to 220.