FIG 1.

European sheatfish virus (ESV) is a close evolutionary relative of epizootic hematopoietic necrosis virus (EHNV). (A) Dot plot analysis of the complete ESV genome compared to EHNV, indicating sites of sequence insertion (solid triangles), deletion (open triangles), and inversion (circle). (B) Phylogenetic tree calculated by the neighbor-joining method using the concatemeric protein sequence corresponding to the 26 core genes of the indicated ranaviruses. STIV, soft-shelled turtle iridovirus; RGV, Rana grylio virus; FV3, frog virus 3; CMTV, common midwife toad virus; TFV, tiger frog virus; SGIV, Singapore grouper iridovirus. Bootstrap percentages (500 replicates) are indicated next to the branches, and the tree is drawn to scale, with distances given as the number of amino acid substitutions per site. Evolutionary analyses were conducted in MEGA6. The positions of ESV and EHNV are indicated by dots.