FIG 4.
MDDCs are more susceptible to HTLV-1 infection with biofilm than activated primary lymphocytes. Primary activated lymphocytes (A) and MDDCs (B) were exposed to biofilm, concentrated virus ([virus]), or supernatant (Sup). Genomic DNA was extracted 3 days postinfection and analyzed by real-time PCR. The percentage of positive cells was normalized according to the amount of HTLV-1 p19gag present in each viral preparation. (C) The same results were plotted for each viral preparation. For panels A to C, the median values from 25 independent experiments obtained with 21 different blood donors are indicated. Asterisks indicate statistically significant differences calculated using a paired t test: ***, P < 0.001; ****, P < 0.0001; ns, not significant. (D) ALU-PCR was carried out on genomic DNA extracted from biofilm-exposed cells (lanes 1 and 4), concentrated virus-exposed cells (lanes 2 and 5), and viral supernatant-exposed cells (lanes 3 and 6). The length of the amplified gag sequence is shown on the right (arrow). DNA extracted from C91PL cells was used as a positive control (lane 8). Negative control, no DNA (lane 7). MW, molecular weight. (E) Results of the ALU-PCR analyses performed on genomic DNA extracted from cells from 5 different blood donors (donors 7, 10, 12, 23, and 30).