FIG 6.
HTLV-1-infected MDDCs transmit the virus to reporter T cells. (A) Schematic representation of the experimental procedure. (B) Real-time PCR performed on genomic DNA extracted from biofilm- or [virus]-exposed MDDCs. Results were normalized according to the amount of p19gag present in each viral preparation. The median values from 5 independent experiments obtained with 5 different blood donors are indicated. Asterisks indicate statistically significant differences calculated using a paired t test: *, P < 0.1; ns, not significant. (C) Luciferase activity was measured after coculture of reporter T cells with biofilm-exposed MDDCs. Noninfected reporter cells were used as a negative control (NI). The median values from 5 independent experiments obtained with 5 different blood donors are indicated. Asterisks indicate statistically significant differences calculated using a paired t test: **, P < 0.01; *, P < 0.1; ns, not significant. (D) Real-time PCR performed on genomic DNA extracts from biofilm-exposed MDDCs in the absence (black bars) or in presence (gray bars) of AZT. Results from independent experiments using cells from 3 different blood donors are represented as percentages of Tax-positive MDDCs in the absence of AZT. (E) Luciferase activity measured after coculture of reporter T cells with biofilm-exposed MDDCs in the absence (black bars) or in presence (gray bars) of AZT. Results from independent experiments using cells from 3 different blood donors are represented as percentages of the luciferase activity obtained with MDDCs in the absence of AZT.