FIG 4.

Inhibition of PKR pathway by MPXV. HeLa cells were either mock infected (lanes 1 and 2) or infected with wt VACV (lanes 3 to 6), VACVΔE3L (lanes 7 to 10), VACV-E3LΔ37N (lanes 11 to 14), or MPXV (lanes 15 to 18) in the presence (+) (lanes 2, 5, 6, 9, 10, 13, 14, 17, and 18) or absence (lanes 1, 3, 4, 7, 8, 11, 12, 15, and 16) of 1,000 U/ml of IFN at an MOI of 5. Protein lysates were isolated at 6 and 9 hpi and analyzed by Western blotting with antibodies specific to the phosphorylated forms of PKR (PKR-P) and eIF2α (eIF2α-P). Detection of GAPDH was used to ensure equal loading of proteins.