FIG 7.
The MPXV F3 protein restores the IFNr phenotype. (A) Through in vitro recombination, the F3L gene of MPXV was inserted into the E3L locus of VACVΔE3L, generating a recombinant VACV that expresses the MPXV F3 protein (VACV-F3L). RK13 cells were treated with increasing amounts of IFN-α A/D for 18 h, prior to infection with 100 PFU of VACV-F3L. At 48 hpi, the cells were stained with crystal violet. (B) Subconfluent BSC-40 cells were treated with the indicated amount of recombinant IFN at 18 hpi. Treated cells were infected with approximately 100 PFU of VACV E3LΔ37N, VACV-F3L, or EMCV. Cells were stained with crystal violet at 48 hpi.