TABLE 3.

Glycosylation on Pr80^enva

Fragment	Mean molecular mass of Pr80env (cytoplasmic) product (kDa) ± SD		Mean estimated glycosylation level (kDa) ± SD
	+ Chymo	+ Chymo + Endo F
Pr	82 ± 4	58 ± 2	24 ± 3
P1	69 ± 3	52 ± 2	17 ± 1
P2	56 ± 2	42 ± 1	14 ± 1
P3	50 ± 2	39 ± 1	10 ± 0
TM	37 ± 3	26 ± 2	12 ± 3
T1	31 ± 0	22 ± 5	9 ± 0
T2	26 ± 9	21 ± 0	6 ± 0

^aHEK 293T cells cotransfected with ΔGP-FLAG and Zfp111 were lysed, and portions of the cell lysate fraction containing Pr80^env (cytoplasm) were treated with chymotrypsin (Chymo) only or chymotrypsin with Endo F, as described in Materials and Methods, and analyzed by SDS-PAGE and Western blotting for FLAG, as shown in Fig. 8A and B. Glycosylation levels for each partial chymotryptic fragment were calculated by measuring the size difference between the glycosylated fragment (chymotrypsin only, e.g., P1) and its respective deglycosylated counterpart (chymotrypsin plus Endo F, e.g., P1*). The values shown are the mean molecular masses and standard deviations from at least 2 independent experiments. All values were rounded to the nearest whole number and are given in kilodaltons. Pr, full-length polypeptide; T1, transmembrane fragment 1.