. 2015 Aug 5;89(20):10453–10466. doi: 10.1128/JVI.01631-15

TABLE 5.

Glycosylation on P70^env^a

Fragment	Mean molecular mass of P70^env (nuclear) product (kDa) ± SD		Mean estimated glycosylation level (kDa) ± SD
Fragment	+ Chymo	+ Chymo + Endo F	Mean estimated glycosylation level (kDa) ± SD
NP	72	62	10
NP1	62	Not detected	Undetermined
NP2	55	Not detected	Undetermined
NP3	50	50	0
NP4	46	46	0
NP5	41	41	0

HEK 293T cells cotransfected with ΔGP-FLAG and Zfp111 were lysed, and the nuclear fraction of the cell lysate fraction containing P70^env (nuclear) was digested with chymotrypsin (Chymo) only or chymotrypsin plus Endo F, analogous to the analysis of Pr80^env (Tables 2 and 3 and Fig. 9A). Glycosylation levels for each fragment were calculated by measuring the difference in the size the glycosylated fragments and the size of their respective deglycosylated counterparts. The numbers shown are the mean molecular masses from two independent treatments. All values were rounded to the nearest whole number and are given in kilodaltons. NP, full-length polypeptide; NP1, polypeptide fragment 1.