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. 2015 Aug 5;89(20):10696–10701. doi: 10.1128/JVI.01430-15

FIG 3.

FIG 3

RNA binding and IFN antagonism by bat IAV NS1 mutants. (A) RNA binding by HL17 NS1 mutants. RNA binding for the indicated V5-tagged HL17 NS1 constructs was performed as described in Fig. 2A. Quantified bound protein (PD, pulldown) was normalized to input, and the bound HL17 NS1 wild type was arbitrarily set to 100%. Bars represent means and standard deviations from three independent experiments. Significance was calculated using Student's t test (****, P < 0.0001; **, P < 0.01; ns, not significant). (B) IFN antagonism by HL17 NS1 mutants. IFN-β reporter assays using the indicated V5-tagged HL17 NS1 constructs were performed as described in Fig. 1A. Significance was calculated using Student's t test comparing the appropriate HL17 NS1 WT dose with each respective mutant dose (***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, not significant). (C) Intracellular localization of HL17 NS1 mutants. Immunofluorescence assays in transfected MRC5-hTERT cells using the indicated V5-tagged HL17 NS1 constructs were performed as described in the legend to Fig. 1D.