FIG 4.
Multicycle replication kinetics of chimeric bat IAVs expressing wild-type or mutant HL17 NS1 proteins in A549 and A549-NPro cells. Cells were infected at a multiplicity of infection of 0.001 PFU/cell with the indicated virus, and supernatants were harvested at various time points prior to titration by plaque assay. (A) Bat-HA/NASC35M expressing wild-type HL17 NS1. (B) Bat-HA/NASC35M expressing HL17 NS1-R39A. (C) Bat-HA/NASC35M expressing HL17 NS1 R39A/K42A. Means and standard deviations from three independent experiments are shown.