FIG 1.

In vitro assembly and purification of VLPs. (a) Diagram of RSV Gag (top) and the extent of the GagΔMBD construct (black rectangle, bottom). GagΔMBD starts at residue 84 of MA. (b) Sedimentation purification of RSV GagΔMBD VLPs. VLPs banded to 40 to 50% (wt/wt) sucrose were separated from unassembled and aggregated protein on a 30 to 60% sucrose step gradient. (c) Negative stain EM image of purified VLPs at low (left) and high (right) magnification (the scale bars are 500 and 100 nm, respectively).