FIG 3.
PrPSc deposition associated with brain ventricles and vasculature. PrPSc IHC (B, D, and F) and hematoxylin and eosin stain (A, C, and E) in adjacent tissue sections of CKY CWD (A and B)- and WST CWD (C to F)-infected SGH. (A and B) Heavy PrPSc deposition associated with the ependymal cell layer (e) of the third ventricle (3). PrPSc plaques (white asterisks and black arrowheads) were located below the ependymal cells, PrPSc extended through this cell layer, and PrPSc projected into the third ventricle. (C and D) A large PrPSc plaque (white asterisk) at the brain surface was found adjacent to PrPSc deposition surrounding an arteriole (a) in the pia. (E and F) Several PrPSc plaques were observed along the midline of the cerebral cortex. Large unicentric and multicentric hematoxylin-positive plaques (white arrowhead and asterisk, respectively) were often observed in WST CWD but were less common in CKY CWD infection of SGH. Brain tissue was from the second (CKY CWD) and third (WST CWD) serial passages in SGH. Scale bar, 20 μm.