FIG 5.
Enrichment and deglycosylation of PrPSc from TME and CWD strains. Brain homogenates from SGH infected with HY TME, DY TME, WST CWD, and CKY CWD were enriched for PrPSc by detergent extraction, ultracentrifugation, and proteinase K digestion (A), and N-linked carbohydrates were removed after enzymatic deglycosylation with PNGase F (B). Brain tissue was from second (CKY CWD) and third (WST CWD) serial passages in SGH. Samples were analyzed by SDS-PAGE and Western blotting with anti-PrP D18 antibody. Short white horizontal lines are located below the aglycosylated polypeptide band of HY TME and placed between each lane for reference purposes.