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. 2015 Aug 5;89(20):10427–10441. doi: 10.1128/JVI.01586-15

FIG 7.

FIG 7

Conformational stability and solubility of prion protein in transgenic mice and Syrian golden hamsters infected with TME and CWD strains. The CSSA was performed using three individual brains from the TME and CWD strains in transgenic mice and SGH. The percentage of insoluble prion protein (PrP) indicates the percentage in the pellet fraction when 100% is equal to the sum of the soluble and pellet fraction for a given [GdnHCl]. The CSSA results for the prion strains in SGH (A) and HPrP7752KO mice (B) are plotted on the same graph. The CSSA results for each individual prion strain in both host species are also plotted on a single panel (E through H). The CSSA results for uninfected SGH and HPrP7752KO mice (C), and the solubility of PrP in N-laurylsarcosine and 0 M GdnHCl from mock-infected and HY TME-infected HrP7752KO brain (D) are also illustrated. Brain tissue was obtained from the second (CKY CWD) and third (WST CWD) serial passages in SGH and from the fourth (CKY CWD) and third (WST CWD) serial passages in HPrP7752KO mice.