TABLE 1.
Incubation period after serial passage of CWD isolates into transgenic mice and Syrian golden hamsters
| Recipient groupa | Inoculumb (incubation period in days) | HPrP7752KO mice |
Syrian golden hamsters |
Total no. of serial passages | ||||
|---|---|---|---|---|---|---|---|---|
| Incubation period (days) ± SEM | A/Ic | Pass no.d | Incubation period (days) ± SEM | A/Ic | Passage | |||
| Control (M6148) | CWD (NA) | 417 ± 23.6 | 4/4 | First | 1 | |||
| WST CWD passage | ||||||||
| M6299 | M6148.1 (369) | 198 ± 3.1 | 5/5 | Second | 2 | |||
| M6339 | M6299.1 (192) | 189 ± 5.7 | 4/4 | Third | 3 | |||
| M6386 | M6339.2 (189) | 187 ± 1.8 | 4/4 | Fourth | 4 | |||
| H1471 | M6299.1 (192) | 379 ± 3.0 | 4/4 | First | 3 | |||
| H1572 | H1471.4 (382) | 343 ± 5.0 | 8/8 | Second | 4 | |||
| H1733 | H1572.1 (340) | 323 ± 2.1 | 19/19 | Third | 5 | |||
| M6429 | H1572.1 (340) | 180 ± 8.3 | 3/3 | First back pass | 5 | |||
| M6595 | M6429.3 (197) | 218 ± 9.1 | 5/5 | Second back pass | 6 | |||
| CKY CWD passage | ||||||||
| M6332 | M6148.3 (473) | 360 ± 16 | 3/3 | Second | 2 | |||
| M6374 | M6332.1 (343) | 160 ± 4.6 | 4/4 | Third | 3 | |||
| M6388 | M6374.4 (151) | 157 ± 0.0 | 3/3 | Fourth | 4 | |||
| H1604 | M6374.4 (151) | 477 ± 15.4 | 3/3 | First | 4 | |||
| H1727 | H1604.2 (468) | 479 ± 6.4 | 20/20 | Second | 5 | |||
| M6448 | H1604.2 (468) | 156 ± 2.5 | 5/5 | First back pass | 5 | |||
| M6596 | M6448.1 (151) | 178 ± 2.5 | 6/6 | Second back pass | 6 | |||
Recipient rodents were either HPrP7752KO mice (indicated by an “M” preceding the recipient group number) or Syrian golden hamsters (indicated by an “H” preceding the recipient group number).
The inoculum was a 1% brain homogenate, a 10% brain homogenate (for initial passage of CWD isolate into HPrP7752KO mice and for inoculation of CKY CWD into Syrian golden hamsters), or a 0.01% brain homogenate (M6148.3). All inoculations were administered i.c. using the inoculum from an individual animal in the recipient group (indicated by the suffixes .1, .2, .3, and .4). The incubation period for the transgenic mouse or Syrian golden hamster used with the inoculum is indicated in parentheses. NA, not applicable.
A/I, number of animals affected by prion disease versus the total number inoculated.
Number of serial passages in either HPrP7752KO mice or Syrian golden hamsters.