The accumulation of immature virions devoid of nucleoid can be observed in Cts2 virus-infected U2OS cells. (A to D) Representative TEM images from WT virus-infected (A) and Cts2 virus-infected (B to D) U2OS cells. Magnifications, ×8,000 (bar = 2 μm) (A) and ×15,000 (bars = 1 μm) (B to D). C, crescent; IV, immature virion; IVN, immature virion with nucleoid; MV, mature virion; D, dense virions. (E) Working model of the involvement of B1 at various stages in the vaccinia virus life cycle.