FIG 4.

Effects of the mR-int330 shRNA insertion on the packaging of spliced RNA in viral particles and reporter expression after transduction. The proportion of spliced RNA packaged into HIV-1 (A) and HTLV-1 (C) particles and the levels of reporter gene expression after transduction of the HIV-1-based (B) and HTLV-1-based (D) vectors containing mR-int330 compared with those obtained after transduction of the corresponding vectors without modification, for which the values were defined as 1, are shown. Results from at least three independent experiments are shown as averages ± standard deviations. *, the value is significantly different from that for the control (P < 0.05, Student's t test).