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. 2015 Aug 12;89(20):10637–10647. doi: 10.1128/JVI.01868-15

FIG 1.

FIG 1

SFV4-miRT124 replicates despite the presence of IFN-I. (A) IFN-β measured from cell culture medium of infected CT-2A-Fluc cells. VA7-EGFP-induced IFN-β was significantly reduced compared to that with SFV4. SFV4-miRT124 induced significantly more IFN-β than VA7-EGFP or SFV4 (P < 0.001). Data are means of three replicates ± SD. (B) Ser396-phosphorylated IRF3 detected from infected or uninfected (mock) CT-2A-Fluc cells by Western blotting. (C) Plaque assay of CT-2A-Fluc cells. Both SFV and SFV4-miRT124 formed plaques in CT-2A-Fluc cultures (under agarose cover). (D) Plaque expansion of VA7-EGFP is increased when virus is administered with Jak inhibitor 1 (inh.1). (E) SFV4 and SFV4-miRT124 potently induce a cytopathic effect in mouse IFN-β-treated CT-2A-Fluc cells. Cells were infected with an MOI of 10. Cell viability was measured in an MTT assay. Data are presented as means (from 5 replicates) ± SD.