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SFV4 nonstructural genes required for IFN-I-resistant replication in CT-2A cells. (A) Schematic of the chimeric viruses we used. Segments marked in black were derived from neurovirulent SFV4. Numbers indicate nucleotide positions. (B) Cell viability was measured in an MTT assay 48 h after infection, with (black bars) or without (white bars) pretreatment with 1,000 units/ml IFN-β. Means ± SD from 4 parallel wells are presented.
