FIG 4.
VA7-EGFP, SFV4, and SFV4-miRT124 showed similar replication potencies in CT-2A-Fluc cells under normal culture conditions. (A) Phase-contrast and fluorescence microscopy images (overlaid) were taken before and 24, 48, and 70 h after infection (MOI, 0.01). (B) Cell viability was measured in an MTT assay 72 h postinfection with an MOI of 0.01. Means of 4 replicates ± SD are presented. (C) Infectious titers were determined 24 h postinfection from culture medium collected from 12-well plates seeded with 4 × 105 CT-2A-Fluc cells. Titers were measured from a pooled sample of three wells infected in parallel for the plaque assay using Vero(B) cells. Means of 3 replicate measurements ± SD are presented.