FIG 5.
Tumor growth and mouse survival. (A) Representative IVIS images of animals treated with SFV4-miRT124 or VA7-EGFP (1 × 106 PFU i.p. at day 3). Signal disappearance can be seen between day 6 and day 9 following SFV4-miRT124 therapy. (B) Representative MRIs of SFV4-miRT124- and VA7-EGFP-treated mice at day 22. A large tumor mass was clearly detectable following VA7-EGFP therapy (arrow). (C) The tumor-emitted luminescence signal was quantified as the average radiance (photons per second per square centimeter per steradian). The increase was measured as the fold change compared to the first measurement, performed at day 2. Data are plotted as geometric means ± SD. Undetectable signals were given a value of 0.1. Statistical analysis was done using an unpaired, two-tailed t test. Comparison results for VA7-EGFP or PBS treatments versus SFV4-miRT124 treatment are marked with stars or circles, respectively: *** or •••, P < 0.001; ** or ••, P < 0.01. (D) Kaplan-Meier survival plot for the mice. Statistical analysis used Fisher's exact test. *. P < 0.05. (E) Comparison of IVIS and MRI signal development in an untreated mouse. (F) Toluidine blue staining of tumor mass in mouse displaying endpoint symptoms.