FIG 5.

Freshly sorted SIV-specific CD8⁺ T cells are more effective at suppressing viral replication in CD4⁺ T cells than macrophages infected with Y223F. A comparison of the MHC-I-specific suppression of viral replication in WT- and Y223F-infected target cells after subtraction of MHC-I-mismatched nonspecific killing is shown. The mean percent suppression of viral replication is represented as a black line, and each data point represents an independent experiment. Cocultures of macrophages infected at a high MOI were performed with Gag_181–189 CM9- and Nef_137–146RL10-CD8⁺ T cells isolated from multiple elite controller animals. Cocultures of low-MOI-infected macrophages included Gag_181–189 CM9- and Vif_66–73HW8-CD8⁺ T cells isolated from infected progressor or vaccinated animals. Two experiments included Nef_137–146RL10-CD8⁺ T cells from an elite controller as effector cells. P values: ***, 0.0001 to 0.001; **, 0.001 to 0.01; *, 0.01 to 0.05.