FIG 6.

Freshly sorted SIV-specific CD8⁺ T cells are more effective at eliminating Δnef-infected CD4⁺ T cells than Δnef-infected macrophages. (a to d) Contour plots representing intracellular p27 staining of MHC-I-matched target cells infected with WT- and Δnef virus as indicated. Contour plots were generated by gating on live CD8⁻ T cells or live CD14⁺ macrophages, and data are from one representative experiment. (e) MHC-I-specific elimination in WT- and Δnef-infected target cells, including macrophages infected at a low MOI, after subtraction of MHC-I-mismatched nonspecific killing. The mean percent elimination of target cells is represented as a black line, and each data point represents an independent experiment. Macrophage cocultures were performed with Gag_181–189 CM9-, Vif_97–104WY8-, Vif_66–73HW8-, or Vif_100–109VL10-CD8⁺ T cells isolated from infected or vaccinated animals and used as effector cells. Cocultures used for the experiments shown in panels a and b or in panels c and d were completed with target cells from the same animal using the same effector cells. P values: ***, 0.0001 to 0.001; **, 0.001 to 0.01; *, 0.01 to 0.05.