. Author manuscript; available in PMC: 2015 Nov 1.

Published in final edited form as: Forensic Sci Int Genet. 2014 May 20;13:20–29. doi: 10.1016/j.fsigen.2014.05.007

Table 2.

Comparison of the two long-range PCR approaches based on quality metrics generated by the NextGENe^® and MiSeq Reporter software platforms.

	NextGENe Analysis
Long-Range PCR Protocol	Input file Read 1 Converted Reads (% of total)	Input file Read 2 Converted Reads (% of total)	Aligned to rCRS Read 1 & 2 (% of total)
Holland average±one stnd dev.	97.8±1.1	90.4±3.8	96.2±4.9
Makova average±one stnd dev.	96.2±3.6	88.3±7.3	87.8±21.9
Welch t-test p-value^*	0.0000	0.0000	0.0002

Two-tailed Welch t-test with 95% Confidence Level