Table 4.
Quality metrics generated by MiSeq Reporter for sequencing runs that used both Qubit and q-PCR methods for quantification and sequencing runs using only Qubit quantification after optimization.
| Quantification Method |
Sample Count |
Cluster (K/m2) |
Yield (Gb) |
>Q3 (%) |
Clusters (K) |
Passing % |
Unaligned (K) |
Unindexed (K) |
Duplicate (K) |
|---|---|---|---|---|---|---|---|---|---|
| Qubit + q− | 12 | 697 | 6.0 | 84.8 | 11,76 | 91.2 | 2,18 | 242 | 3,98 |
| 12 | 307 | 2.9 | 87.6 | 5,54 | 91.5 | 1,77 | 147 | 1,06 | |
| 11 | 634 | 5.8 | 89.7 | 11,23 | 91.6 | 4,09 | 180 | 2,19 | |
| 11 | 666 | 6.2 | 87.0 | 11,90 | 91.9 | 3,46 | 222 | 2,91 | |
| 11 | 1107 | 8.1 | 80.4 | 15,73 | 79.1 | 4,82 | 505 | 4,12 | |
| 11 | 991 | 7.7 | 81.1 | 15,03 | 82.0 | 5,30 | 355 | 3,53 | |
| 11 | 1323 | 10.5 | 82.8 | 20,47 | 83.4 | 7,21 | 377 | 5,58 | |
| 11 | 1181 | 9.7 | 82.5 | 18,95 | 85.7 | 5,41 | 376 | 5,18 | |
| 12 | 550 | 5.0 | 89.6 | 9,77 | 91.8 | 173 | 463 | 3,20 | |
| 12 | 863 | 7.7 | 88.3 | 14,89 | 89.9 | 336 | 691 | 5,72 | |
| avg. | 854. | 7.0 | 84.5 | 13633. | 87.0 | 3549. | 353. | 3454. | |
| stnd. | 317. | 2.3 | 3.5 | 4416. | 4.8 | 2323. | 167. | 1498. | |
| Qubit | 12 | 992 | 8.7 | 86.6 | 16,90 | 89.3 | 421 | 761 | 6,51 |
| 11 | 986 | 8.5 | 84.9 | 16,60 | 88.6 | 5,73 | 319 | 4,44 | |
| avg. | 989. | 8.6 | 85.8 | 16754. | 89.0 | 3077. | 540. | 5482. | |
| stnd. | 4.2 | 0.1 | 1.2 | 212. | 0.5 | 3756. | 312. | 1465. |