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. Author manuscript; available in PMC: 2015 Sep 23.
Published in final edited form as: Ultramicroscopy. 2009 Mar 28;109(8):1094–1104. doi: 10.1016/j.ultramic.2009.03.043

Figure 3.

Figure 3

Nanoscale, three-dimensional contour map of protein assembly within the neuronal porosome complex. Top Left: Atomic force micrograph of an immunoisolated neuronal porosome, reconstituted in lipid membrane. Note the central plug of the porosome complex and the presence of approximately 8 globular units arranged at the lip of the complex. Top Right: Negatively stained electron micrographs of isolated neuronal porosome protein complexes. Note the 10–12 nm complexes exhibiting a circular profile and having a central plug. Approximately 8–10 interconnected protein densities are observed at the rim of the structure, which are connected to a central element via spoke-like structures. Bottom Left: Electron density maps of negatively stained electron micrographs of isolated neuronal porosome protein complexes. Bottom Right: 3D topography of porosomes obtained from their corresponding electron density maps. The colors from yellow, through green to blue, correspond to the protein image density from lowest to the highest. The highest peak in each image represents 27 Å.