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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Jan 31;1853(5):904–917. doi: 10.1016/j.bbamcr.2015.01.015

Fig. 3.

Fig. 3

Knockdown of VLDLR significantly decreases synaptophysin puncta number. (A–B) Mouse brain lysates were immunoprecipitated with IgG or VLDLR (IIII) followed by an immunoblot with synaptophysin (A) and PSD-95 (B). (C) Primary hippocampal neurons (DIV12) were transfected with GFP + PLL or GFP + VLDLR shRNA for 3 days. Cells were then fixed and immunostained with Synaptophysin (left) or PSD-95 (right). (D) Quantification of synaptophysin puncta number in C (PLL n=6 neurons, VLDLR shRNA n=7 neurons, *p<0.05). (E) Quantification of PSD-95 puncta number in C (PLL n=6 neurons, VLDLR shRNA n=8 neurons) (F) Primary hippocampal neurons (DIV18) were transfected with GFP + PLL or GFP + VLDLR shRNA for 3 days. Cells were then fixed and immunostained with Synaptophysin (left) or PSD-95 (right). (G) Quantification of synaptophysin puncta number in F (PLL n=5 neurons, VLDLR shRNA n=7 neurons, *p<0.05). (H) Quantification of PSD-95 puncta number in F (PLL n=8 neurons, VLDLR shRNA n=8 neurons).