(A) Immunostaining of in vitro cultures of primary skeletal myoblasts treated with si-Snai1, si-Snai2, or si-Scrambled (control). Depletion of Snai1 or Snai2 results in precocious differentiation of myoblasts under growth conditions as shown by a significant increase in the number of myosin heavy-chain (MyHC)-expressing cells (red) in the si-Snai1- or si-Snai2-treated myoblasts compared to the control. Nuclei were counterstained with Hoechst (green).
(B) Real-time RT-PCR with gene-specific primers showing the extent of knockdown of Snai1 and Snai2 compared to si-Scrambled (control).
(C) Quantification of MyHC expressing myoblasts from (A) compared to the baseline spontaneous differentiation of a control from (A). Error bars represent standard deviation (n = 3). See also Figure S4.