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. 2015 Sep 22;15:384. doi: 10.1186/s12879-015-1125-3

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© Vallur et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Optimization of antigen capture ELISA using affinity purified and labeled antibody pair. a. Standard curve was generated using pooled urine from NEC spiked with 0, 4, 8, 16 and 32 ng/mL of Ld WCL for determining limits of detection of the antibody pair. b. Performance of capture ELISA on urine samples from VL patients (n = 29), OD (n = 7) and NEC (n = 8). * and ** represent significant p-values < 0.05, as calculated by One-way ANOVA