Skip to main content
. 2015 Sep 20;23(9):724–733. doi: 10.1089/ars.2015.6265

FIG. 2.

FIG. 2.

Defective plasma membrane targeting of mutant HA-DUOX2 C124G coexpressed with myc-DUOXA2. (A) Representative histograms of flow immunocytofluorometry experiments. The upper panel shows that MG132 (2 μM) does not change the absence of expression of mutant HA-DUOX2 C124G at the cell surface. The middle panel shows that mutant HA-DUOX2 affects the cell surface targeting of myc-DUOXA2 in the presence or in the absence of MG132. The bottom panel shows that intracellular expression of myc-DUOXA2 in cells permeabilized with saponin is decreased in the presence of mutant HA-DUOX2, but MG132 rescues the inhibition. ***P<0.001. (B) Surface expression of WT or mutant HA-DUOX2 C124G in permeabilized HEK293 cells cotransfected with myc-DUOXA2. Magnification×63. The nuclei were stained withDAPI. Note that mutant DUOX2 and its partner are condensed in a perinuclear zone (C) Localization of DUOX2 and DUOXA2 proteins at the apical surface of human thyrocytes analyzed by immunohistochemistry. Human thyrocytes were analyzed for DUOX2 expression under reducing and nonreducing conditions. HA, hemagglutinin.