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. 2015 Sep 23;13:80. doi: 10.1186/s12915-015-0186-5

Table 4.

Composition of protein bound and free intracellular amino acids of P. pastoris grown on glucose or methanol/glycerol in chemostats at μ = 0.1 h−1

Protein bound amino acids Free intracellular amino acids
Methanol/glycerol Glucose Log2FC Methanol/glycerol Glucose Log2FC P value
Average [mg/gCDW] Average [mg/gCDW] Average [mg/gCDW] Average [mg/gCDW]
Asx 48.8 30.2 0.69 5.42 2.91 0.89 0.00
Ala 32.1 20.2 0.67 0.90 1.02 −0.18 0.28
Arg 29.6 25.9 0.19 11.1 12.1 −0.12 0.47
Cys 5.59 4.56 0.30
Glx 87.0 68.0 0.36 22.3 29.4 −0.40 0.00
Gly 18.7 11.5 0.70
His 11.9 7.33 0.70 0.76 0.74 0.03 0.78
Ile 17.8 10.6 0.76 0.05 0.04 0.28 0.00
Leu 36.0 22.6 0.67 0.10 0.08 0.35 0.00
Lys 37.5 24.5 0.62 0.76 0.92 −0.28 0.18
Met 5.34 4.33 0.30 0.04 1.08 −4.63 0.00
Phe 20.0 12.9 0.63 0.03 0.03 0.03 0.56
Pro 0.92 2.76 −1.58 0.00
Ser 25.9 19.0 0.44 0.25 0.52 −1.04 0.00
Thr 28.2 19.3 0.55 0.24 0.27 −0.14 0.42
Tyr 17.3 8.89 0.96 0.06 0.05 0.18 0.02
Val 26.4 15.7 0.75 0.25 0.14 0.83 0.00
Total amino acids [mg/g Cell dry weight (CDW)] 448 306 0.55 43.2 52.1 −0.27 0.03
Total protein content [mg/gCDW] 540 390 0.47