Table 5.

Glycerophospholipid, non-polar lipid (TG, triacylglycerol; SE, steryl esters), unesterified ergosterol, and free and total fatty acid content in total cell extracts of Pichia pastoris grown on glucose (GAP) or methanol (AOX) as the sole carbon source. Data are listed as μg lipid/mg Wet Cell Weight which have been calculated from at least two independent experiments with standard deviation (±). Significance was estimated by Student’s t-test (two tailed, unpaired)

	Glucose	Methanol/glycerol	P value
Glycerophospholipids	8.07 ± 0.14	8.92 ± 0.30	0.01
Non-polar lipids
TG	3.16 ± 0.71	1.47 ± 0.28	0.06
SE	0.22 ± 0.03	0.30 ± 0.03	0.07
Free ergosterol	1.67 ± 0.06	1.83 ± 0.18	0.20
Free fatty acids	2.11 ± 0.49	3.38 ± 0.72	0.09
Total fatty acids
C16:0	1.22 ± 0.03	1.43 ± 0.16	0.01
C16:1	0.75 ± 0.01	0.85 ± 0.06	0.003
C18:0	0.33 ± 0.02	0.34 ± 0.09	0.67
C18:1	4.28 ± 0.18	3.30 ± 0.31	0.0002
C18:2	3.26 ± 0.14	3.45 ± 0.30	0.21
C18:3	0.96 ± 0.04	1.30 ± 0.11	0.0001
∑ of fatty acids	10.79 ± 0.40	10.67 ± 1.01	0.79