Table 2. Primers used for PCR of 10 Lip family genes from M. tuberculosis.
| Gene | Primer direction | Primer sequence (5’→3’) | Amplicon Size(bp) |
|---|---|---|---|
| lipI | Sense | CCGGAATTCGCCCAGTTTGGACAACACCGCC | 958 |
| (rv1400c) | Antisense | CCGGTTAACTCCGTGTAGCACAACCCGTAGCG | |
| lipL | Sense | CCGGAATTCGATGGTTGACACCGGAGTCGATCA | 1285 |
| (rv1497) | Antisense | CCGGTTAACGCCCGCCGCGGCTCC | |
| lipM | Sense | CCGGAATTCGGGCGCTCCTCGACTCATCCA | 1291 |
| (rv2284) | Antisense | CCGGTTAACTGGGATCGCAACCGCGGGG | |
| lipN | Sense | CCGGAATTCGACCAAGAGTCTGCCAGGTGTG | 1126 |
| (rv2970c) | Antisense | CCGGTTAACAACCCGGCTAAGGTGCGCG | |
| lipQ | Sense | CCGGAATTCGCACATCGCCAGCGTGACTTCG | 1261 |
| (rv2485c) | Antisense | CCGGTTAACGCTGGCCGGAGGTGACGACA | |
| lipT | Sense | CCGGAATTCGGCCCTGGAGTCGGCTAC | 1531 |
| (rv2045c) | Antisense | CCGGTTAACATTCGCCAGCGAAAACCCGTC | |
| lipU | Sense | CCGGAATTCGGCGGTCCGGCCGGTGCTA | 889 |
| (rv1076) | Antisense | CCGGTTAACCCCGGTGGCCTCGCGGATGTA | |
| lipV | Sense | CCGGAATTCGCCCGAAATCCCCATCGCC | 670 |
| (rv3203) | Antisense | CCGGTTAACGCGCGGTCCCAGTCGACT | |
| lipY | Sense | CGCCATATGTGTCTTATGTTGTTGCGTTGCC | 1309 |
| (rv3097c) | Antisense | CCGGTTAACGGCGGCGATACCGAGTTG | |
| lipZ | Sense | CCGGAATTCGACATCACCGAGTGTCCGGGAG | 862 |
| (rv1834) | Antisense | CCGGTTAACGTCGACGAGCAGCGATAGCGC |
The restriction sites integrated into the sequences are underlined.