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. 2015 Sep 23;10(9):e0137610. doi: 10.1371/journal.pone.0137610

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© 2015 Morita et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Cell extracts were subjected to western blotting using anti-phosphorylated ERK (P-ERK) and anti-ERK (ERK) antibodies. (a) Basal phosphorylation level of ERK in HeLa cells seeded at 1.5 x 10⁴ and 0.5 x 10⁴ cells/cm². (b) Effects of U0126 treatment (1 μM, 24 h or 48 h) and EGF stimulation (10 ng/ml, 5min) in HeLa cells seeded at 1.5 x 10⁴.