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. 2015 Sep 21;25(18):2430–2434. doi: 10.1016/j.cub.2015.07.071

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

OPN4L Mediates Negative Masking

(A) Representative actograms from animals given intravitreal bilateral injection (indicated by a red star) of siNT, siOpn4, siOpn4S, and siOpn4L show negative masking after a 10 min ZT12.5 light pulse (indicated by a red arrow) every day following injection. This protocol avoids significant light-induced phase shifts. Negative masking is attenuated with siOpn4 and siOpn4L. Actograms are enlarged around light pulse for clarity.

(B) Histogram of reduction in activity during the light pulse (as compared with activity preceding the light pulse) across 3-day bins as indicated on the y axis.

(C) Histogram of reduction in average (of days 2–8) wheel-running activity during the nocturnal light pulse shows smaller reductions in activity with siOpn4 (11.1 ± 1.6 versus 22.2 ± 2.3 siNT) and siOpn4L-treated animals (9.7 ± 3.6). siOpn4S had no significant effect (18.0 ± 3.4). n = 6. ^∗p < 0.05; one-way ANOVA with Dunnett’s post-test. Error bars represent the SEM.

See also Figures S1–S4.