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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Anal Bioanal Chem. 2015 Aug 9;407(24):7263–7273. doi: 10.1007/s00216-015-8918-5

Fig. 4.

Fig. 4

ELISA inhibition curves for TCSG using A) Reagent concentrations: antiserum 2621, 1:8,000 (final dilution in wells); coating antigen TCSG-BSA, 4 µg/mL (100 µL/well), secondary Ab – GAR- HRP, 1:10,000 dilution. Coefficient values: Amax 0.88, Amin 0.44, IC50 510.9, slope 0.31 B) 5-BSA as the coating antigen: Heterologous format; reagent concentration: antiserum 2621, 1:5,000 (final dilution in wells); coating antigen - 1 g/mL (100 µL/well), secondary Ab – 1:5,000 dilution. Coefficient values: Amax 0.82, Amin 0.07, IC50 2.85, slope 1.18. Standards prepared in 10% MeOH-PBS (5% MeOH-PBS as final concentration in wells). Each data point represents the mean of quadruplicates.