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. 2015 Sep 23;14:125. doi: 10.1186/s12933-015-0288-y

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© Liu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — A Columns in this figure demonstrated the IL-1β level in supernatant of cultured primary myocytes by ELISA. According to different treatments, equal amount myocytes were divided into Ctrl (control), Scramble (cells transfected with scramble siRNA), HG (cells incubated by medium with glucose concentration at 33 mmol/L), HG + IRAK2^−/− (cells treated with high-glucose medium and transfected with siRNA targeting irak2), HG + IL-1β (cells treated with high-glucose medium and exogenous IL-1β) and HG + IL-1β + IRAK2^−/− (cells treated with high-glucose medium and exogenous IL-1β and transfected with siRNA against irak2) respectively [asterisk differences were significant when compared with HG (p < 0.05]. B Columns in this part showed the detected IL-1β in serum and myocardial tissue supernatant collected from normal control (Ctrl), DCM rats (DCM) and DCM rats received IL-1Ra administration (DCM + IL-1Ra) respectively [letter a differences were significant when compared with Ctrl (p < 0.05)]