Figure 2.

Cell growth and clonogenic survival of HCC1937 breast cancer cell line. Cells were seeded and grown for 7 days in the presence of PF-548 (5 μM), D1(A12) (200 nM), Ab17 (0.5 μg ml⁻¹) or corresponding vehicle controls. (A) Colonies formed by individual cells were stained and counted. (B) The total colony area was also calculated for each biological replicate, and representative images of single colonies are included. *P<0.05, **P<0.001 and ***P<0.0001 compared with vehicle control (Student's t-test). (C) Live cells were counted at three different time points with Trypan blue stain. Percentage cell growth compared with initial seeding density is reported. *P<0.05, ***P<0.0001 compared with IgG at corresponding time point (two-way ANOVA).