Table 2. Primary antibodies.
Antigens | Primary antibody source | Clone | Retrieval buffer | Dilution | References |
---|---|---|---|---|---|
MAGE-A1 | Santa Cruz Biotechnology (Santa Cruz, CA, USA) | MA454 | Tris EDTA | 1 : 50 | (Jungbluth et al, 2000) |
MAGE-A3/4 | Professor G.C. Spagnolia | 57B | Tris EDTA | 1 : 100 | (Landry et al, 2000) |
MAGE-A10 | Professor G.C. Spagnolia | 3GA11 | Citric acid | 1 : 10 | (Schultz-Thater et al, 2011) |
NYESO-1 | Santa Cruz Biotechnology | E978 | Tris EDTA | 1 : 50 | (Vaughan et al, 2004) |
SSX-2 | Professor A.G. van Kesselb | E3AS | Tris EDTA | 1 : 25 | (dos Santos et al, 2000) |
MAGE-C1 | Santa Cruz Biotechnology | CT7-33 | Tris EDTA | 1 : 50 | (Xia et al, 2013) |
MAGE-C2 | Professor Boquan Yinc | CT-10 | Tris EDTA | 1 : 100 | (Zhuang et al, 2006) |
MUC-1 | Sanbio (Uden, The Netherlands) | MA695 | Citric acid | 1 : 100 | (Langner et al, 2004) |
AFP | Dako | Polyclonal | Tris EDTA | 1 : 400 | Dakod |
GPC-3 | Santa Cruz Biotechnology | 1G12 | Tris EDTA | 1 : 200 | (Shirakawa et al, 2009) |
Annexin-A2 | BD Biosciences (Breda, The Netherlands) | 5 | Tris EDTA | 1 : 200 | (Yee et al, 2007) |
WT-1 | Novus Biologicals (Abingdon, UK) | 6F-H2 | Tris EDTA | 1 : 400 | (Nakatsuka et al, 2006) |
Survivin | Santa Cruz Biotechnology | D-8 | Tris EDTA | 1 : 50 | (Brennan et al, 2008) |
MDK | GeneTex (Irvine, CA, USA) | EP1143Y | Citric acid | 1 : 400 | (Liang et al, 2013) |
SP17 | Proteintech (Manchester, UK) | Polyclonal | Citric acid | 1 : 100 | Proteinteche |
SALL-4 | Santa Cruz Biotechnology | EE-30 | Tris EDTA | 1 : 50 | (Yong et al, 2013b) |
MAGE-A3/A4 and MAGE-A10 antibodies graciously provided by Professor Giulio Spagnoli, Department of Surgery, Research Laboratory, University Hospital Basel, Basel, Switzerland (Landry et al, 2000; Schultz-Thater et al, 2011).
SSX-2 antibody graciously provided by Professor Ad Geurts van Kessel, Department of Human Genetics University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands (dos Santos et al, 2000).
MAGE-C2 antibody graciously provided by Professor Boquan Yin, Department of Immunology, Fourth Military Medical University, Xi'an 710032, PR China (Zhuang et al, 2006).
http://www.dako.com/nl/ar38/p102130/prod_products.htm Accessed 8-9-14.
http://www.ptglab.com/PView/SPA17-Antibody-13367-1-AP-PVIEW.htm Accessed 8-9-14.