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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Mar 23;34(50):6040–6054. doi: 10.1038/onc.2015.52

Fig. 7. ERBB3 and HBEGF are required for YAP to regulate ovarian cell proliferation.

Fig. 7

a) HBEGF and NRG1β1 stimulate proliferation of TOV21G cells; b) HBEGF and NRG1β1stimulate proliferation of KGN cells; c) HBEGF and NRG1β1 stimulate the growth of HOSE cell in a 3D hanging drop culture system. The diameter of the well ring is 1mm. d) Ki67 staining (green) in the control, HBEGF-treated and NRG1β1-treated microtissues derived from HOSE cell lines. Nuclei was stained with DAPI (blue); actin was stained with rhodamin-phalloidin. Scale bar; 20μm. e) Knockdown of HBEGF and ERBB3 compromised YAP-stimulated HOSE cell proliferation. f) Knockdown of HBEGF and ERBB3 blocked YAP-stimulated TOV21G cancer cell proliferation. All experiments were repeated at least three times and the representative images were presented. Each bar in bar graphs represents mean ± SEM. Bars with different letters are significantly different from each other (p < 0.05).