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. 2015 Sep 23;10(9):e0138458. doi: 10.1371/journal.pone.0138458

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© 2015 Lei et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — A) Schematic representation of epitope replacements in Bet. Conserved motifs are given schematically as black boxes numbered according to [58]. Modifications based on sequence similarity are marked as “sim” and modifications based on position are marked as “end”. Original protein sequences are shown in black; modifications in red. Epitope sequences are underlined. B) CrFK and C) KE-R cells were infected with virus-containing supernatants harvested from 293T cells transfected with modified and wild-type proviruses. Supernatants were passaged onto uninfected cells every two days. Viral titers were determined by titration on FeFAB cells. Cells were mock-infected with supernatant from pcDNA-transfected cells as a negative control. Titers are presented as mean values of three independent experiments. Error bars represent standard deviation of mean values.